Composite

Part:BBa_K876005:Design

Designed by: Alec Spinhirne   Group: iGEM12_UT_Dallas   (2012-09-30)

pLac-RBS-GFP-T-pTet-LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2211
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 873


Design Notes

This part was designed to simulate one side of biobrick BBa_K876001 in order to determine the efficacy of IPTG binding to LacI and LacI binding to LacO.

Source

This mechanism was built with biobricks submitted to the registry in previous years.

References